Chemistry and Biochemistry

Adam T. Woolley

Adam Woolley

Office: C305 BNSN
Office Phone: 801-422-1701
Lab Phone: 801-422-1397
Email: awoolley@chem.byu.edu
Office Hours

Education:

BS, Brigham Young University (1992)

Ph.D., University of California, Berkeley (1997)

Runyon-Winchell Postdoctoral Fellow, Harvard University (1998-2000)

Curriculum Vitae

Research:

The Woolley Research Group

Micro-and Nanometer-Scale Chemical Manipulation and Analysis:

My group works at the interface between chemistry, engineering and biology. Thus, students receive broad technical training and are well poised to contribute in these key research fields. A common theme in my research is the interrelationship between biological molecules and miniaturization. We are utilizing miniaturization tools to detect and quantify clinically relevant biomolecules, and we are also applying DNA in forming nanoscale materials.

A. Integrated microfluidic systems for preterm birth risk assessment. Preterm birth (PTB) is a serious issue, with approximately 10% of pregnancies resulting in a preterm delivery, frequently coupled with complications that lead to poor outcomes and increased medical costs. We are developing microfluidic systems that combine extraction, fluorescent labeling and separation all in a single microchip (Fig. 1). These devices will provide high-throughput, point of care screening from a finger stick quantity of blood to assess risk of a preterm delivery, weeks before contractions begin.

B. Biotemplated nanofabrication of electronics: My group is leading an interdisciplinary team whose objective is to explore bottom-up methods for the fabrication of nanoscale electronic systems. We fold DNA into controlled nanoscale designs that can be converted into functional electronic elements after purification and metallization (Fig. 2). We are presently applying these methods in making metal-semiconductor junctions with linewidths as small as 5 nm.

C. Rapid Blood Infection Determination: We are developing methods for detecting bacterial infections in blood in less than one hour, in collaboration with a group of biologists and engineers. A schematic of the proposed system is shown in Figure 3. Our focus is on the capture and fluorescent labeling of nucleic acid material from bacteria. We are developing microfluidic systems with solid supports designed to selectively capture nucleic acid sequences from pathogenic organisms in blood. The retained nucleic acids will then be labeled fluorescently for subsequent single-molecule detection.

       
Figure 1. A few microliters of unlabeled blood serum are loaded on a microfluidic device that has all assay reagents preloaded in the other reservoirs. After integrated analysis functions are complete, the concentrations of PTB biomarkers will be known, providing risk assessment and enabling medical care to be implemented. (A) Multiplexed immunoaffinity extraction module. (B) Enrichment, labeling and purification module. (C) Separation module.      

Figure 2. DNA origami are formed with seeding nanoparticles, purified to remove defects, and functionalized with inorganic electronic materials.

   

Figure 3. (Bottom) Schematic of integrated pathogen detection cartridge showing each component. (Top) Schematic of the disposable blood filtration system that delivers bacteria to the port on the cartridge.  

Publications:

Gates, E.P.; Jensen, J.K.; Harb, J.N.; Woolley, A.T. Optimizing Gold Nanoparticle Seeding Density on DNA Origami. RSC Advances 5, 8134-8141 (2015).
 
Yang, R; Pagaduan, J.V.; Yu, M.; Woolley, A.T. On Chip Preconcentration and Fluorescence Labeling of Model Proteins by Use of Monolithic Columns: Device Fabrication, Optimization, and Automation. Anal. Bioanal. Chem. 407, 737-747 (2015).
 
Chatterjee, D.; Mansfield, D.S.; Woolley, A.T. Microfluidic Devices for Label-Free and Non-Instrumented Quantitation of Unamplified Nucleic Acids by Flow Distance Measurement. Anal. Meth. 6, 8173-8179 (2014).
 
Gates, E.P.; Dearden, A.M.; Woolley, A.T. DNA-templated Lithography and Nanofabrication for the Fabrication of Nanoscale Electronic Circuitry. Crit. Rev. Anal. Chem. 44, 354-370 (2014).
 
Uprety, B.; Gates, E.P.; Geng, Y.; Woolley, A.T.; Harb, J.N. Site-specific Metallization of Multiple Metals on a Single DNA Origami Template. Langmuir 30, 1134-1141 (2014).
 
Rogers, C.I.; Oxborrow, J.B.; Anderson, R.R.; Tsai, L.-F.; Nordin, G.P.; Woolley, A.T. Microfluidic Valves Made From Polymerized Polyethylene Glycol Diacrylate. Sens. Actuators B 191, 438-444 (2014).
 
Kumar, S.; Xuan, J.; Lee, M.L.; Tolley, H.D.; Hawkins, A.R.; Woolley, A.T. Thin-Film Microfabricated Nanofluidic Arrays for Size-Selective Protein Fractionation. Lab Chip 13, 4591-4598 (2013).
 
 
 
The Woolley group's article, "Development of an integrated microfluidic solid-phase extraction and electrophoresis device," was featured on the cover of the March 2016 issue of Analyst. 
 

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Awards: