Chemistry and Biochemistry

Bio Analysis Lab

The aim of the Biological Mass Spectrometry and Cell Sorting Core Facility is to provide top tier data acquisition and sample analysis in the fields of proteomics, metabolomics, lipidomics, and flow cytometry to the BYU community. If you are interested in using the facilities mass spectrometry capabilities, please contact Dr. Daniel Mortensen to get started. If you are interested in cell sorting, please contact Research Assistant Max Beers.

Dr. Daniel Mortensen (Director) 

Mass Spectrometry

E114 BNSN
(801) 422-7123
dnmort@chem.byu.edu

Maxwell Wright (Research Assistant)

Cell Sorting

(717) 462-3500

maxswright33@gmail.com


Instrumentation

Mass Spectrometers: (E150 BNSN)

Flow Cytommetry Cell Sorter: (E267 BNSN)

Orbitrap Fusion Lumos Tribrid Mass Spectrometer

(ThermoFisher Website)

FACS Aria Fusion

(BD Biosciences Website)

6530 Accurate-Mass Q-TOF LC/MS

(Agilent Technologies Website)


Pricing and Procedures

These are users prices after university contributions. Prices for users not affiliated with Brigham Young University are negotiated on a per case basis

Mass Spectrometry

Cell Sorting

Use of HPLC/MS or HPLC/MS/MS


$3 per hour for clean samples

$15 per hour for potentially messy samples (such as lipid samples)

Technician Run Data Acquisition or Data Analysis: $12 per hour

* All times are rounded up to the nearest 1/2 hour

Policies and Procedures

  1. If you are interested in using the Bio Mass Spec Facility, please contact Dr. Daniel Mortensen, the facility director, by phone at 801-422-7123 or by email at dnmort@chem.byu.edu.
  2. Use of instrumentation in the Bio Mass Spec Facility is charged on an hourly rate and generally requires a technician to run samples.
  3. Samples will not be analyzed unless they are accompanied by a fully completed worklist. The worklists can be downloaded here:
    1. Orbitrap
    2. Q-ToF
  4. Suggested Methodsfor sample preparation are provided bellow:
    1. On-Filter Trypsin Digest
    2. In Gel Sample Prep for LCMS Sequencing
  5. If data acquired and or analyzed in this facility is included in a publication, financial support from the Fritz B. Burns Foundation should be acknowledged. For example: “Mass spectrometry was supported by the Fritz B. Burns Foundation (2016 gift to Brigham Young University).”
  • Use of the BD FACS Aria Fusion is charged on a per use basis. Each use costs $50 with a maximum annual cost of $1200 per year. This means that users that use the instrument more than 24 times within a single year only pay for the first 24 uses in that year.
  • If you are interested in using the BD FACS Aria Fusion, please contact Max Wright, the head assistant for the facility, by by email at: maxswright33@gmail.com.


PeakInvestigator®

 This critical post-processing of raw scan files significantly extends the researcher’s ability to get the most out of their mass spec results. 

 PeakInvestigator® mass spectral analysis software operates on the researcher’s current (or previously captured) mass spec raw scan file outputs, to maximize extraction and capture of the most real data vs. noise, providing up to 5-6x higher resolution with detection and deconvolution of overlapped peaks, and statistical confidence intervals on every peak call.

PeakInvestigator post-processing of your raw scan files typically provides visibly superior centroided results that can transform the efficiency of downstream MS analyses and identifications, for less invested time and money.

You can access PeakInvestigator software services within either of these open source mass spectral analysis software packages available in this MS core facility:

➢     MZmine PeakInvestigator Edition (MZminePI)

➢     OpenMS PeakInvestigator Edition (OpenMSPI)

If you are not already familiar with PeakInvestigator, you can request a free demo on your data by contacting the MS core facility manager.