Chemistry and Biochemistry

NMR Lab


Contact

Scott Burt
Office: C414/C008A BNSN
Office Phone: 801-422-2404
Lab Phone: 801-422-2054
Email: srburt@chem.byu.edu


Instrument Information

 

 

 

The NMR facility supports both research and teaching in the department. We currently have three high-field superconducting NMR instruments: a Varian INOVA 300 MHz, a Varian INOVA 500 MHz and a Varian NMR-System 500 MHz. Each instrument has pulsed-field gradients, variable-temperature accessories, and multinuclear capabilities. All of our instruments run Agilent's VnmrJ software. Instrument access is tied to the department network accounts to allow users to store their data in a centralized location, regardless of which instrument they use.

 

 
 
  

 

 

 

 

 

Inova 300 MHz System – This instrument has a quad-nucleus probe, allowing straightforward acquisition of 1H, 13C, 31P and 19F spectra (i.e. no need to swap tuning inserts or change the tuning/matching capacitors). Pulsed-field gradients are available and reduce artifacts in certain experiments. A wide variety of experiments are available on this instrument, but the most common are standard 1D, DEPT, COSY, TOCSY, HSQC, H2BC, HMBC and NOESY. Band-selective 1D and 2D experiments are also available. A broadband probe is available, allowing detection of most NMR active nuclei. However, low-frequency nuclei typically benefit from higher field instruments.  
 
 
 
 
 
 
 
 
 
 
 
Inova 500 MHz System – This instrument has a double-resonance probe optimized for X-detection and a triple-resonance probe optimized for 1H detection. The default configuration is 1H/13C, but tuning inserts can be used to observe essentially any nucleus of interest. Pulsed-field gradients are available and reduce artifacts in certain experiments. A wide variety of experiments are available on this instrument, but the most common are standard 1D, DEPT, COSY, TOCSY, HSQC, H2BC, HMBC and NOESY. Band-selective 1D and 2D experiments are also available. A 10 mm broadband probe is also available.
 
 
 
 
 
 
 
 
 
 
 
 
 
NMR-System 500 MHz System – This instrument has an auto-tuning OneNMR probe.  This probe has enhanced 1H RF homogeneity and sensitivity that greatly improves the quality of inverse-detected 2D NMR experiments.  Furthermore, the auto-tuning capabilities provide simple access to many NMR active nuclei (e.g., 1H, 7Li, 11B, 13C, 15N, 17O, 19F, 27Al, 29Si, 31P, 133Cs, 195Pt, etc.) Pulsed-field gradients are available and reduce artifacts in certain experiments. A wide variety of experiments are available on this instrument, but the most common are standard 1D, DEPT, COSY, TOCSY, HSQC, H2BC, HMBC and NOESY. Band-selective 1D and 2D experiments are also available. 

 

 

 

   

 

 

 

 

 

Data Processing Computers – These computers are available for off-line processing of your NMR data. The software is identical to that provided on the instrument computers and includes tools for analyzing multiple data sets at the same time. Dual monitors are provided to improve the use of multi-viewport analysis of your NMR data.

 

 

 

 

 


Policies/Training

You must complete the appropriate NMR training in order to to obtain access to the NMR facility and the individual instruments.  Please contact Dr. Burt if you need training.  The following training modules are available:

Introduction to 1D & 2D NMR - This training is required to obtain access to the facility and the instruments. Topics covered are sample preparation, instrument preparation (locking/shimming), acquiring basic 1D and 2D experiments, walkup automation tools, and processing the NMR data. Experiments discussed are 1D Hydrogen, 1D Carbon, COSY (1H-1H correlation), and HSQC (13C-1H correlation).

Advanced 1D NMR - This training addresses more advanced techniques, such as DEPT, solvent suppression, 1D-NOESY, 1D-TOCSY, and nuclei other than 13C and 1H.  Probe matching/tuning as well as manual locking/shimming are also covered in this training.

Advanced 2D NMR - The emphasis in this training module is small molecule structure determination using modern inverse-detected experiments as well as the multiple viewport tools available in VnmrJ.  Experiments discussed are DQF-COSY, HSQC, H2BC, HMBC, NOESY as well as variations such as band-selective experiments and hyphenated experiments (e.g. HSQC-TOCSY).

Misc Topics - Other specialized topics can be addressed via one-on-one training.  Examples include variable temperature operation, diffusion resolved spectroscopy, etc.

Correlation with Chemistry Courses - Note that the training received in Chem 455 is equivalent to Intro to 1D & 2D NMR.  The Chem 555 training covers the same training as Chem 455 as well as Advanced 1D NMR.  Chem 655 covers the same material as Advanced 2D NMR.


Instrument Scheduling

You must schedule time on the instrument using the Chemistry Department Resource Scheduling Page.  You will not be able to schedule the instrument until you have completed your basic NMR training


User Tools

Last updated 10 February 2015